Constitutive oligomerization of PAR-2 reduces membrane turnover. (A) Two-cell embryos expressing the indicated mNG::PAR-2(S241A) alleles or membrane tethered GFP::PAR-2(S241A)+PH::GBP were subject to targeted photobleaching (∼0.4 s) along one edge of the anterior AB cell (highlighted regions, A). Images show the entire embryo before bleaching (A, top, scale bar = 10 µm) and insets highlighting the bleached zone for the indicated times relative to photobleaching (A, bottom, scale bar = 5 µm). (B) Quantification of membrane fluorescence within the center of the bleached region (A, dashed lines) shown relative to a corresponding unbleached control region and normalized to pre-bleach intensity. Mean shown along with individual replicates (n indicated). Note that reduced turnover correlates with better segregation (4mer > 2mer > GBP > WT, compare to segregation data in Fig. 7).