Reducing membrane turnover anteriorizes posterior protein PAR-2. (A) Mutation of the PKC-3 target site in PAR-2, PAR-2(S241A), leads to modest transient anterior enrichment of PAR-2. Note reversed asymmetry compared to PAR-2(WT). The magnitude and stability of anterior segregation is enhanced by tethering to the membrane (PH::GFP) or by oligomerization (2mer, 4mer). Representative midplane confocal images captured at the end of the establishment phase (End of flow) and maintenance phase (Post NEBD) highlighting peak segregation and degree of asymmetry decay once flows cease. (B) Quantification of membrane profiles of zygotes expressing PAR-2(S241A) variants shown in A. Mean ± SD shown. (C and D) Quantification of ASI and relative posterior depletion for PAR-2(S241A) variants plotted along with Post NEBD data for PAR-3 zygotes for comparison (see Fig. 4). Note that both oligomeric forms of PAR-2(S241A, 2mer/4mer) achieve near maximal levels for both ASI (0.5) and posterior depletion (1.0) during the establishment phase, which decay only minimally once flows cease. Data for S241A are pooled from GFP:: and mNG::PAR-2(S241A), which behaved identically.