PAR-6 undergoes weak and transient asymmetry when decoupled from PAR-3 clusters. Representative midplane confocal images of PAR-6::GFP in wild-type or a pkc-3(ts) background in which PAR-6 membrane association is decoupled from PAR-3. When PAR-6 is not associated with PAR-3 clusters, segregation still occurs but is weaker and more transient.