Figure S2.
FRAP analysis of bulk PAR-3 turnover on the membrane. Two-cell embryos expressing mNG::PAR-3 were subject to targeted photobleaching (∼0.4 s) along one edge of the anterior AB cell (highlighted regions, A). (A and B) Images show the entire embryo before bleaching (A, scale bar = 10 µm) and insets highlighting the bleached zone for the indicated times relative to photobleaching (B, scale bar = 5 µm). (C) Quantification of membrane fluorescence within the center of the bleached region (B, dashed lines) shown relative to a corresponding unbleached control region and normalized to pre-bleach intensity. Mean ± SD shown (n = 6). Data fit to a one-component association model to obtain the characteristic turnover time, τ (1/koff), shown with 95% CI.

FRAP analysis of bulk PAR-3 turnover on the membrane. Two-cell embryos expressing mNG::PAR-3 were subject to targeted photobleaching (∼0.4 s) along one edge of the anterior AB cell (highlighted regions, A). (A and B) Images show the entire embryo before bleaching (A, scale bar = 10 µm) and insets highlighting the bleached zone for the indicated times relative to photobleaching (B, scale bar = 5 µm). (C) Quantification of membrane fluorescence within the center of the bleached region (B, dashed lines) shown relative to a corresponding unbleached control region and normalized to pre-bleach intensity. Mean ± SD shown (n = 6). Data fit to a one-component association model to obtain the characteristic turnover time, τ (1/koff), shown with 95% CI.

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