Figure S3. Supplemental in vitro... | Rockefeller University Press

Figure S3.

Supplemental in vitro analysis of the effect of Zn2+on kinesin and dynein motor activity, and in situ investigation of ATP/ADP levels in COS-7 cells. (A and B) Representative microtubule-stimulated ATPase activity showing non-normalized ATPase activity per motor domain per second for purified (A) recombinant human kinesin (KIF5A) or (B) a minimally processive, artificially dimerized yeast dynein fragment (GST-dynein331) across a range of Zn2+ concentrations (from same dataset as Fig. 5, A and B). (C) Mean ATP:ADP ratio (±SEM) in COS-7 cells as measured by PercevalHR across baseline, 100 µM ZnCl2 and 2.5 µM pyrithione, washout, and 2.5–5 µM oligomycin treatment (representing seven cells from three individual replicates).

Supplemental in vitro analysis of the effect of Zn ²⁺ on kinesin and dynein motor activity, and in situ investigation of ATP/ADP levels in COS-7 cells. (A and B) Representative microtubule-stimulated ATPase activity showing non-normalized ATPase activity per motor domain per second for purified (A) recombinant human kinesin (KIF5A) or (B) a minimally processive, artificially dimerized yeast dynein fragment (GST-dynein₃₃₁) across a range of Zn²⁺ concentrations (from same dataset as Fig. 5, A and B). (C) Mean ATP:ADP ratio (±SEM) in COS-7 cells as measured by PercevalHR across baseline, 100 µM ZnCl₂ and 2.5 µM pyrithione, washout, and 2.5–5 µM oligomycin treatment (representing seven cells from three individual replicates).