Figure S2. Zn 2 influx inhibits motility... | Rockefeller University Press

Figure S2.

Zn2+influx inhibits motility of lysosomes and mitochondria in HeLa cells. (A and C) Representative lysosomal (A) and mitochondrial (C) motility (determined using the Total Motility plugin for Fiji; see Materials and methods) in HeLa cells treated with 20 µM ZnCl2 and 1.25 µM pyrithione (PTO), then washed and treated with 100 µM TPEN (treatments added at time points indicated on plots). (B and D) Mean (±SEM) lysosomal (B) and mitochondrial (D) motility normalized to their respective baselines (n = 4 and 5 cells, from four to three independent replicates, respectively). One-way repeated measures ANOVA, with post-hoc Tukey HSD. (E) Representative traces of lysosomal motility in HeLa cells stained with LysoTracker Red and treated with 2.5 µM pyrithione and either 20 µM CuCl2 (teal lines) or 20 µM ZnCl2 (black line) at 0 sec. All experiments were performed in the absence of extracellular Ca2+. *** P < 0.0001, ** P < 0.01, * P < 0.05, n.s. not significant.

Zn ²⁺ influx inhibits motility of lysosomes and mitochondria in HeLa cells. (A and C) Representative lysosomal (A) and mitochondrial (C) motility (determined using the Total Motility plugin for Fiji; see Materials and methods) in HeLa cells treated with 20 µM ZnCl₂ and 1.25 µM pyrithione (PTO), then washed and treated with 100 µM TPEN (treatments added at time points indicated on plots). (B and D) Mean (±SEM) lysosomal (B) and mitochondrial (D) motility normalized to their respective baselines (n = 4 and 5 cells, from four to three independent replicates, respectively). One-way repeated measures ANOVA, with post-hoc Tukey HSD. (E) Representative traces of lysosomal motility in HeLa cells stained with LysoTracker Red and treated with 2.5 µM pyrithione and either 20 µM CuCl₂ (teal lines) or 20 µM ZnCl₂ (black line) at 0 sec. All experiments were performed in the absence of extracellular Ca²⁺. *** P < 0.0001, ** P < 0.01, * P < 0.05, n.s. not significant.