Figure S5.
Representative photobleaching steps for GFP-Cdt1, SKA1/2Cy3,and Ndc80alexa647. (A–C) Representative photobleaching steps for indicated proteins. Histogram (B) of starting fluorescence intensity of indicated single molecules and photobleaching steps (C) from n > 100 single molecule binding events and N = 3 independent trials.

Representative photobleaching steps for GFP-Cdt1, SKA1/2 Cy3 , and Ndc80 alexa647 . (A–C) Representative photobleaching steps for indicated proteins. Histogram (B) of starting fluorescence intensity of indicated single molecules and photobleaching steps (C) from n > 100 single molecule binding events and N = 3 independent trials.

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