Kymographs for dynamic microtubule plus-ends tracking by Ska1, Cdt1, and Ndc80 when present as indicated combinations. (A–D) Additional example kymographs for dynamic microtubule end tracking by one fluorescently tagged component when mixed with the other component(s) as indicated on top of each panel, bar 1 min, 5 μm. (E) Representative time lapse images recorded with surface immobilized microtubules after addition of a mixture of the untagged whole Ndc80 complex (1 nM), GFP-tagged SKA1/2/3 hexameric complex (1 nM), soluble tubulin (1 mg/ml), and Mg-GTP (1 mM). Time is in s, and bar is 5 µm. To the right is a kymograph of the dynamic plus-ends tracking by the indicated component. (F) Additional example kymographs for dynamic microtubule end tracking by the indicated proteins on top of each panel; bars, 1 min, 5 μm. (G) Representative time lapse images recorded with surface immobilized microtubules after addition of a mixture of HEC1/NUF2-GFP, untagged SKA1/2 (1 nM), soluble tubulin (1 mg/ml), and Mg-GTP (1 mM). Time is in s, and bar is 5 µm. To the right is a kympgraph of the dynamic plus-ends tracking by the indicated component. (H) More example kymographs for dynamic microtubule end tracking by GFP-Cdt1, untagged SKA1/2, and Ndc80^alexa647. Panels showing kymographs for the same end-tracking events is GFP-Cdt1 channel, and in Ndc80^alexa647 channel.