Figure 2. Staufen impairs oskar mRNA... | Rockefeller University Press

Figure 2.

$Staufen impairs oskar mRNA transport and dynein activation in vitro. (A) Kymographs (time-distance plots) exemplifying the behavior of oskar RNA and dynein in the presence (filled circle) and absence (open circle) of Staufen; in both conditions, Egl, BicD, and dynactin are also present but not fluorescently labeled. Minus end is to the left and plus end to the right. (B–G) Quantification of motile properties of oskar mRNA (B–D) and dynein (E–G) in the conditions shown in A. Charts show frequency of processive movements (B and E), total number of microtubule (MT) binding events (C and F), and fraction of microtubule-binding events that result in processive motility (D and G). Plots show the mean ± SD of values from 15 individual microtubules (represented by black circles) derived from analysis of 586–1,341 single RNA particles (B–D) or 1,247–2,207 single dynein particles (E–G) per condition. Statistical significance and P-values were determined with Mann–Whitney tests. (H–I) Quantification of effect of Staufen on number of processive dynein complexes and fraction of dynein-microtubule binding events that result in processive motility for motor activated by dynactin, oskar RNA, and Egl/BicD (H) vs. motor activated by dynactin and BicD2N (I). Values for conditions with Staufen were normalized to the corresponding condition without Staufen to obtain relative metrics. Plots show the mean ± SD of values from 10 individual microtubules (represented by black circles) derived from analysis of 536-925 single dynein particles per condition. Statistical significance and P-values were determined with Brown-Forsythe and Welch ANOVA tests.$

Staufen impairs oskar mRNA transport and dynein activation in vitro. (A) Kymographs (time-distance plots) exemplifying the behavior of oskar RNA and dynein in the presence (filled circle) and absence (open circle) of Staufen; in both conditions, Egl, BicD, and dynactin are also present but not fluorescently labeled. Minus end is to the left and plus end to the right. (B–G) Quantification of motile properties of oskar mRNA (B–D) and dynein (E–G) in the conditions shown in A. Charts show frequency of processive movements (B and E), total number of microtubule (MT) binding events (C and F), and fraction of microtubule-binding events that result in processive motility (D and G). Plots show the mean ± SD of values from 15 individual microtubules (represented by black circles) derived from analysis of 586–1,341 single RNA particles (B–D) or 1,247–2,207 single dynein particles (E–G) per condition. Statistical significance and P-values were determined with Mann–Whitney tests. (H–I) Quantification of effect of Staufen on number of processive dynein complexes and fraction of dynein-microtubule binding events that result in processive motility for motor activated by dynactin, oskar RNA, and Egl/BicD (H) vs. motor activated by dynactin and BicD2N (I). Values for conditions with Staufen were normalized to the corresponding condition without Staufen to obtain relative metrics. Plots show the mean ± SD of values from 10 individual microtubules (represented by black circles) derived from analysis of 536-925 single dynein particles per condition. Statistical significance and P-values were determined with Brown-Forsythe and Welch ANOVA tests.