Figure 9.
IST1 structures are not reticular adhesions. (A) STED micrographs of moDCs immunolabeled for IST1 (magenta) and tubulin (green). Scale bar: 10 μm. (B) Confocal micrographs of moDCs immunolabeled for IST1 (magenta), vinculin or talin (cyan), and phalloidin (yellow). Blue: DAPI. Bar graphs show PCC ± SD (n = 3 donors). PCC values were compared to 0 using one sample t test. (C) Confocal micrographs of moDCs immunolabeled for CHMP1 (magenta) and the reticular adhesion markers NUMB, DAB2 or clathrin light chain (cyan) and phalloidin (yellow), and DAPI (blue). For clathrin light chain, the image showing a higher z-plane indicates that clathrin labeling was successful. PCC values were compared to 0 using one sample t test (n ≥ 3 donors). Scale bars: 10 μm. For the statistical analysis of B and C, data distribution was assumed to be normal, but this was not formally tested. Scale bars: 10 µm.

IST1 structures are not reticular adhesions. (A) STED micrographs of moDCs immunolabeled for IST1 (magenta) and tubulin (green). Scale bar: 10 μm. (B) Confocal micrographs of moDCs immunolabeled for IST1 (magenta), vinculin or talin (cyan), and phalloidin (yellow). Blue: DAPI. Bar graphs show PCC ± SD (n = 3 donors). PCC values were compared to 0 using one sample t test. (C) Confocal micrographs of moDCs immunolabeled for CHMP1 (magenta) and the reticular adhesion markers NUMB, DAB2 or clathrin light chain (cyan) and phalloidin (yellow), and DAPI (blue). For clathrin light chain, the image showing a higher z-plane indicates that clathrin labeling was successful. PCC values were compared to 0 using one sample t test (n ≥ 3 donors). Scale bars: 10 μm. For the statistical analysis of B and C, data distribution was assumed to be normal, but this was not formally tested. Scale bars: 10 µm.

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