Figure S4.
IST1 structures do not colocalize with caveolin-1 and the ALIXΔPRR mutant. (A) More examples for the GPI-anchored RFP shown in main Fig. 6 B. TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta in merge) with GPI-anchored RFP (green). (B) TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta in merge) with mCherry-tagged caveolin 1 (green). (C) TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta) with mCherry-tagged ALIXΔPRR mutant (green) or only mCherry (control). Graph shows the average number of IST1-positive structures for cells with detectable expression of both constructs for three different donors (paired two-sided t test). For the statistical analysis, data distribution was assumed to be normal, but this was not formally tested. Scale bars: 10 μm, unless indicated otherwise.

IST1 structures do not colocalize with caveolin-1 and the ALIXΔPRR mutant. (A) More examples for the GPI-anchored RFP shown in main Fig. 6 B. TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta in merge) with GPI-anchored RFP (green). (B) TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta in merge) with mCherry-tagged caveolin 1 (green). (C) TIRF microscopy of moDCs co-expressing GFP-labeled IST (magenta) with mCherry-tagged ALIXΔPRR mutant (green) or only mCherry (control). Graph shows the average number of IST1-positive structures for cells with detectable expression of both constructs for three different donors (paired two-sided t test). For the statistical analysis, data distribution was assumed to be normal, but this was not formally tested. Scale bars: 10 μm, unless indicated otherwise.

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