Figure S2.
LPS does not influence the IST1 structures and they are not present in non-migratory non-adhering cell types. (A) moDCs were cultured in the presence of absence of 0.1 μg/ml LPS for 4 h and immunolabeled for IST1. Graphs show the average number of IST1-positive structures per cell and the average size of the structures. Each dot-line-dot pair represents a donor (n = 3; paired two-sided t tests were performed; NS = not significant; data distribution was assumed to be normal, but this was not formally tested). (B) Confocal micrographs of the indicated cell types immunolabeled for IST1 (magenta). A z-plane close to the coverslip is shown. Green: phalloidin. Blue: DAPI. Murine neurons, microglia and astrocytes were cultured for 6 h, whereas the other cells were cultured for 2 h. Murine microglia were also immunolabeled for the microglia marker CD11b (yellow). Scale bars: 10 μm.

LPS does not influence the IST1 structures and they are not present in non-migratory non-adhering cell types. (A) moDCs were cultured in the presence of absence of 0.1 μg/ml LPS for 4 h and immunolabeled for IST1. Graphs show the average number of IST1-positive structures per cell and the average size of the structures. Each dot-line-dot pair represents a donor (n = 3; paired two-sided t tests were performed; NS = not significant; data distribution was assumed to be normal, but this was not formally tested). (B) Confocal micrographs of the indicated cell types immunolabeled for IST1 (magenta). A z-plane close to the coverslip is shown. Green: phalloidin. Blue: DAPI. Murine neurons, microglia and astrocytes were cultured for 6 h, whereas the other cells were cultured for 2 h. Murine microglia were also immunolabeled for the microglia marker CD11b (yellow). Scale bars: 10 μm.

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