Figure 3.
IST1 structures contain other ESCRT components. (A) Confocal micrographs of monocyte-derived dendritic cells (moDCs) immunolabeled for IST1 (magenta in merge) and the indicated proteins (green). Blue: DAPI. The line graphs show fluorescence intensity profiles as indicated by the yellow lines. The yellow shaded areas indicate regions where the fluorescence intensity of both proteins exceeds the 50% intensity. The bar graphs show the PCC ± SD (n ≥3 donors, except VPS4 for which we only succeeded to obtain n = 1; see B). PCC values were compared to 0 using one sample t tests. Data distribution was assumed to be normal, but this was not formally tested. *: P < 0.05; **: P < 0.01; NS: not significant. (B) MoDCs transfected with VPS4-GFP and immunolabeled for GFP (green) and IST1 (magenta; n = 3 donors). Scale bars: 10 μm.

IST1 structures contain other ESCRT components. (A) Confocal micrographs of monocyte-derived dendritic cells (moDCs) immunolabeled for IST1 (magenta in merge) and the indicated proteins (green). Blue: DAPI. The line graphs show fluorescence intensity profiles as indicated by the yellow lines. The yellow shaded areas indicate regions where the fluorescence intensity of both proteins exceeds the 50% intensity. The bar graphs show the PCC ± SD (n ≥3 donors, except VPS4 for which we only succeeded to obtain n = 1; see B). PCC values were compared to 0 using one sample t tests. Data distribution was assumed to be normal, but this was not formally tested. *: P < 0.05; **: P < 0.01; NS: not significant. (B) MoDCs transfected with VPS4-GFP and immunolabeled for GFP (green) and IST1 (magenta; n = 3 donors). Scale bars: 10 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal