Figure 7. Recovery of other autophagy... | Rockefeller University Press

Figure 7.

$Recovery of other autophagy proteins in membrane fractions and in GFP-LC3B immunoprecipitations. (A) Immunoblots of cell membrane fractions from GFP-LC3B expressing WT cells (left) and GFP-LC3B expressing ATG2 DKO cells (right) showing enrichment of autophagy factors in WT fractions 5 and 6 and in ATG2 DKO fraction 5. Cells were grown in indicated conditions as in Fig. 3. Loaded protein: 5 µg. (B) Immunoblots of GFP IPs performed as in Fig. 6 B. Input protein for each IP: 50 µg. Loaded input protein: 5 µg (10%). Loaded IPs: 10% of the total collected beads.$

Recovery of other autophagy proteins in membrane fractions and in GFP-LC3B immunoprecipitations. (A) Immunoblots of cell membrane fractions from GFP-LC3B expressing WT cells (left) and GFP-LC3B expressing ATG2 DKO cells (right) showing enrichment of autophagy factors in WT fractions 5 and 6 and in ATG2 DKO fraction 5. Cells were grown in indicated conditions as in Fig. 3. Loaded protein: 5 µg. (B) Immunoblots of GFP IPs performed as in Fig. 6 B. Input protein for each IP: 50 µg. Loaded input protein: 5 µg (10%). Loaded IPs: 10% of the total collected beads.