The pre-ATG2 compartment is an organized collection of pre-autophagosomal proteins and membranes. Line scans corresponding to six individual LC3B-positive structures from ATG2 DKO HEK293 cells per early autophagy factor. (A) ATG9A signal largely colocalizes with LC3B but in the largest structures is also found enriched in the compartment core. (B) p62 consistently colocalizes with LC3B with strongest signal in the center of the compartment in the majority of sampled cells. (C) WIPI2 signal is consistently found on the outside of the LC3B signal, colocalizing with LC3B at the rim of the compartment. (D) FIP200 colocalizes with LC3B, with both direct overlap and adjacent localization. Inset images are single slices from confocal Z-stacks. Inset scale bars: 3 µm.