Figure S3.
shVAMP4 knocks down VAMP4 expression in PC12 cells across varied tagBFP expression levels. For Fig. 3. (A) Representative confocal slices of PC12 cells expressing shScramble-tagBFP immunostained for endogenous VAMP4 (anti-VAMP4) and tag-BFP (anti-tagBFP) after 48 h Dox treatment. Cells with different levels of tagBFP staining all show an intracellular pool of VAMP4 (yellow arrows). (B) Representative confocal slices of PC12 cells expressing shVAMP4-tagBFP under similar experimental conditions as A. Compared to the cells with no anti-tagBFP staining (far-left panel), cells with varied levels of tagBFP staining all lose the VAMP4 pool. Corresponding channels of all images are contrasted to the same. Scale bar = 20 µm.

shVAMP4 knocks down VAMP4 expression in PC12 cells across varied tagBFP expression levels. For Fig. 3. (A) Representative confocal slices of PC12 cells expressing shScramble-tagBFP immunostained for endogenous VAMP4 (anti-VAMP4) and tag-BFP (anti-tagBFP) after 48 h Dox treatment. Cells with different levels of tagBFP staining all show an intracellular pool of VAMP4 (yellow arrows). (B) Representative confocal slices of PC12 cells expressing shVAMP4-tagBFP under similar experimental conditions as A. Compared to the cells with no anti-tagBFP staining (far-left panel), cells with varied levels of tagBFP staining all lose the VAMP4 pool. Corresponding channels of all images are contrasted to the same. Scale bar = 20 µm.

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