PC12 cells express detectable endogenous VAMP2, but (our) HEK293 cells do not. For Fig. 3. (A) Immunoblot of VAMP2 from the whole-cell lysates of untransfected HEK293 cells (plain HEK), HEK293 cells transfected with VAMP2-pHuji (HEK + VAMP2), and untransfected PC12 cells (plain PC12). Whole-cell lysates were each prepared from one well of confluent 6-well plate. Three replicates of lysates preparation are shown. (B) Representative confocal images showing that there is no VAMP2 immunostaining in plain HEK293 cells. The images in the VAMP2 channels of PC12 and HEK293 were acquired under similar conditions and were adjusted to the same levels. DAPI staining shows the nuclei to confirm that there are many cells in the field. Scale bar = 10 µm. (C) Protein sequence alignment between human VAMP2 isoform1 (hVAMP2 isoform1) and rat VAMP2 (rVAMP2) shows close similarity. Source data are available for this figure: SourceData FS2.