Kip2-NMD binds to SPBs and tracks microtubule plus-ends by binding to Bik1. (A) Representative images of preanaphase cells expressing Spc42-mCherry (magenta) and Kip2-NMD-3xsfGFP (green) or Kip2-NMD-ΔT-3xsfGFP (green) in the context of control, bik1Δ, or kip3Δ. (B) Quantification of Kip2-NMD-3xsfGFP or Kip2-NMD-ΔT-3xsfGFP localization at preanaphase SPBs and aMT plus-ends in control and mutant cells. Cells from three independent clones were quantified, n as shown in the graph. (C) Kip2-NMD-mNeonGreen accumulates on both the growing and shrinking cytoplasmic microtubule plus-ends. The red arrowhead marks the plus-end. SPBs are visualized with Spc72-GFP. The time-lapse movie has 1.07 s intervals, shown as Video 2; only representative time points are shown here. (D) Representative images of preanaphase heterozygous diploid cells expressing Kip2-G374A-3xsfGFP (green) and the wild-type protein Kip2-mCherry (magenta). Line scan analysis of the two aMTs as highlighted in yellow boxes on the right. All scale bars, 2 µm.