Experimental and estimate results of Kip2 distribution along microtubules. (A–F) Quantifications of fluorescence intensities (a.u.) from endogenous Kip2-∆T-3xsfGFP and Kip2-3xsfGFP in the presence or absence of Bik1 along preanaphase cytoplasmic microtubules binned by microtubule length: (A) 0.80–1.06 μm, (B) 1.06–1.33 μm, (C) 1.33–1.60 μm, (D) 1.60–1.86 μm, (E) 1.86–2.13 μm, (F) 2.13–2.40 μm. (G–I) Experimental Kip2-∆T-3xsfGFP (H), Kip2-3xsfGFP in the presence (G) or absence (I) of Bik1 fluorescence (a.u.) mean profile (black) and standard error (gray) with respective mean in silico estimation fits (red) for microtubules binned by length. Red dashed lines past plus-end and SPB indicates estimation extrapolations without support by data. (J) In silico kymograph with on rate constant of 6.1E-4 (nM Kip2)⁻¹ s⁻¹, in rate constant of 3.1E-1 (nM Kip2)⁻¹ s⁻¹, off rate constant of 2.3E-2 s⁻¹, and total Kip2 concentration of 35 nM.