The Kip2–Bik1 interaction is not required for efficient targeting of Kip2, but of Bik1, to microtubule plus-ends. (A) Representative kymographs drawn from time-lapse series of preanaphase cells of the indicated genotype. SPBs were visualized with Spc72-GFP. Red arrowheads denote the speckles that appeared along the shaft of microtubules. (B) Quantification of Kip2 speckle moving speed using the kymographs shown in A. More than 90 speckles analyzed per condition. (C) Measurements of Bik1-3xGFP fluorescence intensity (%) on cytoplasmic microtubule plus-ends in cells of the indicated genotype. More than 90 cells analyzed per condition. (D) Western blot analysis of endogenously expressed Kip2-6HA and Kip2-ΔT-6HA. Lysates were prepared from cycling cells of the indicated genotype. (E) Representative images of preanaphase cells expressing the SPB marker Spc42-mCherry (magenta) and the ATPase deficient variant Kip2-G374A-3xsfGFP (green) in the presence and absence of Bik1. (F) Measurements of Kip2-G374A-3xsfGFP fluorescence intensity (%) associated with b-SPBs in cells shown in E. More than 90 speckles cells per condition. Statistical significance was calculated using two-tailed Student's t test. **** P < 0.0001; n.s., not significant. Source data for B, C, and F are available in Data S1. Source data are available for this figure: SourceData FS2.