Figure S3.
Soluble tubulin does not abolish CLASP’s depolymerase activity until the tubulin concentration is above the critical concentration for microtubule growth. (A) Representative kymographs of microtubules in the presence of 0, 4 and 8 µM soluble tubulin and 200 nM CLASP1. The stable microtubule seed is shown in magenta and the tubulin is shown in green. (B) Quantification of the microtubule plus-end polymerization rate across a range of tubulin concentrations from 0 to 8 µM in the presence of 200 nM CLASP1. Data are means ± SE obtained over three experimental days. (C) An example kymograph of a microtubule undergoing CLASP-mediated depolymerization at one end and polymerization at the other in the presence of 8 µM soluble tubulin and 200 nM CLASP1.

Soluble tubulin does not abolish CLASP’s depolymerase activity until the tubulin concentration is above the critical concentration for microtubule growth. (A) Representative kymographs of microtubules in the presence of 0, 4 and 8 µM soluble tubulin and 200 nM CLASP1. The stable microtubule seed is shown in magenta and the tubulin is shown in green. (B) Quantification of the microtubule plus-end polymerization rate across a range of tubulin concentrations from 0 to 8 µM in the presence of 200 nM CLASP1. Data are means ± SE obtained over three experimental days. (C) An example kymograph of a microtubule undergoing CLASP-mediated depolymerization at one end and polymerization at the other in the presence of 8 µM soluble tubulin and 200 nM CLASP1.

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