Purification of proteins used in this study. (A) SDS-page gel showing purified His-chTOG, His-CLASP1, His-CLASP2α, His-CLASP2γ, and His-EGFP-L-TOG2-S proteins. The lower band in the His-EGFP-L-TOG2-S sample likely represents a truncated protein or breakdown product as no significant contaminating proteins were found in the mass spectrometry analysis. (B) Mass spectrometry analysis of His-CLASP1 protein. (C) Mass spectrometry analysis of His-EGFP-L-TOG2-S protein. The hits with a total spectrum count of 10 or more are listed. Source data are available for this figure: SourceData FS1.