Figure 8.
RacC regulates cortical actin assembly via interaction with Fbp17 and WASP. (A) Localization of RFP-RacCG15V and GFP-Fbp17 in randomly migrating cells. (B) Localization of RFP-RacCG15V and GFP-WASP in randomly migrating cells. (C–E) Time-lapse imaging of GFP-RacCG15V and RFP-ArpC4 in WT (C), fbp17− (D), and wasA− (E) cells during random migration. Angle-series plots at the bottom show fluorescent intensity distribution of the indicated proteins along the perimeter of the cell, with 0° and +180°/−180° corresponding to the migrating front and rear, respectively. Solid lines represent the mean and shades represent mean ± SD. n, number of cells analyzed. Scale bars, 5 μm.

RacC regulates cortical actin assembly via interaction with Fbp17 and WASP. (A) Localization of RFP-RacCG15V and GFP-Fbp17 in randomly migrating cells. (B) Localization of RFP-RacCG15V and GFP-WASP in randomly migrating cells. (C–E) Time-lapse imaging of GFP-RacCG15V and RFP-ArpC4 in WT (C), fbp17− (D), and wasA− (E) cells during random migration. Angle-series plots at the bottom show fluorescent intensity distribution of the indicated proteins along the perimeter of the cell, with 0° and +180°/−180° corresponding to the migrating front and rear, respectively. Solid lines represent the mean and shades represent mean ± SD. n, number of cells analyzed. Scale bars, 5 μm.

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