GxcM interacts with the F-BAR protein Fbp17. (A) Proteomic identification of Fbp17 as a binding partner of GxcM. (B) Schematic representation of full-length Fbp17 and truncation constructs. (C) Co-IP of GxcM-GFP or GxcM4A-GFP with RFP or RFP-Fbp17. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. (D) Co-IP of the indicated GFP-fusion proteins with RFP-Fbp17. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. (E) Co-IP of GxcM-GFP with the indicated RFP-fusion proteins. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. (F) Pull-down of GxcM-GFP from cell lysate with GST or GST-SH3 immobilized on beads. Samples were probed with GFP antibody. The protein-transferred membrane was stained with Ponceau S to show purified GST and GST-SH3. (G) Pull-down of the indicated GFP-fusion proteins from cell lysate with GST-SH3 immobilized on beads. Samples were probed with GFP antibody. The protein-transferred membrane was stained with Ponceau S to show purified GST-SH3. (H) Pull-down of GxcM-GFP from cell lysate with GST-SH3 or MBP-SH3 immobilized on beads. Samples were probed with GFP antibody. The protein-transferred membrane was stained with Ponceau S to show purified GST and MBP fusion proteins. (I) Co-IP of GFP-Fbp17 or GFP-Fbp17F-BAR with RFP or RFP-Fbp17. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. (J) Co-IP of the indicated GFP-fusion proteins with RFP-Fbp17. IP was performed with RFP-trap and samples were probed with GFP or RFP antibody. Source data are available for this figure: SourceData F3.
