Figure 2.
GxcM overexpression induces cortical actin assembly. Time-lapse imaging of WT cells migrating under agarose along a folic acid gradient. (A) Cells expressing LimEΔcoil-RFP and GFP. (B) Cells expressing LimEΔcoil-RFP and GxcM-GFP. (C) Cells expressing GFP-ArpC4 and RFP. (D) Cells expressing GFP-ArpC4 and GxcM-RFP. Angle-series plots on the right show fluorescent intensity distribution of the indicated proteins along the perimeter of the cell, with 0° and +180°/−180° corresponding to the migrating front and rear, respectively. Solid lines represent the mean and shades represent mean ± SD. n, number of cells analyzed. The white triangles indicate gradient direction. Scale bars, 5 μm.

GxcM overexpression induces cortical actin assembly. Time-lapse imaging of WT cells migrating under agarose along a folic acid gradient. (A) Cells expressing LimEΔcoil-RFP and GFP. (B) Cells expressing LimEΔcoil-RFP and GxcM-GFP. (C) Cells expressing GFP-ArpC4 and RFP. (D) Cells expressing GFP-ArpC4 and GxcM-RFP. Angle-series plots on the right show fluorescent intensity distribution of the indicated proteins along the perimeter of the cell, with 0° and +180°/−180° corresponding to the migrating front and rear, respectively. Solid lines represent the mean and shades represent mean ± SD. n, number of cells analyzed. The white triangles indicate gradient direction. Scale bars, 5 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal