A comparison of superimposed time courses. Mean time traces (data from Figs. 5 and 8) from ER, mitochondria, and cytosolic sensors in WT and a Rtnl1 mutant (Rtnl1^-), and in Type Is and Ib excitatory synapses. Plots are normalized so that the difference of minimum to maximum ΔF/F values are set to 100 for the WT recordings in each compartment. Most time courses are from 40 Hz stimulation trials. Two exceptions are: the Ib cytosolic trace was with 50 Hz stimulation, as 40 Hz was not tested, Is ER responses were with 20 Hz, as this frequency was most representative of the lack of statistical difference between WT and Rtnl1 mutant responses (Fig. 6 D).