LAMP1ΔYQTI-RUSH is glycosylated during RUSH assay. GFP immunoblot showing increasing LAMP1Δ-RUSH glycosylation over time after biotin addition. Note that LAMP1Δ-RUSH starts showing glycosylation from 20 min onwards. Gly = glycosylated LAMP1Δ-RUSH, Non-gly = non-glycosylated LAMP1Δ-RUSH.