Figure S10. Rtnl1 loss decreases... | Rockefeller University Press

Figure S10.

Rtnl1 loss decreases cytosolic Ca2+handling in Type Ib boutons. (Extended data from Fig. 5.) (A) Impact of Rtnl1 loss of function on peak evoked cytosolic Ca2+. Plots show all single time traces and mean ± SEM of time traces in both genotypes for the four stimulation frequencies tested. Sample size (larvae) is indicated within the plot for each genotype. (B and C) Rtnl1 loss of function does not affect time to peak cytosolic Ca2+, or time to 50% recovery. (D) Rtnl1 loss of function decreases time to 100% recovery in every stimulation frequency tested. In B–D, plots show mean ± SEM of every genotype for each stimulation frequency tested; sample size (larvae) is indicated within the plot for each genotype. For each larva, we analyzed responses from a 20-s recording from one muscle 1 NMJ between segments A4-A6. Datapoints represent the time to peak ΔF/F and 50% or 100% recovery. Comparisons were analyzed with a mixed-effects two-way ANOVA. Genotypes are Ib-GAL4, UAS-myrGCaMP6s/UAS-tdTom::Sec61β, in either a WT or Rtnl118 background.

Rtnl1 loss decreases cytosolic Ca ²⁺ handling in Type Ib boutons. (Extended data from Fig. 5.) (A) Impact of Rtnl1 loss of function on peak evoked cytosolic Ca²⁺. Plots show all single time traces and mean ± SEM of time traces in both genotypes for the four stimulation frequencies tested. Sample size (larvae) is indicated within the plot for each genotype. (B and C) Rtnl1 loss of function does not affect time to peak cytosolic Ca²⁺, or time to 50% recovery. (D) Rtnl1 loss of function decreases time to 100% recovery in every stimulation frequency tested. In B–D, plots show mean ± SEM of every genotype for each stimulation frequency tested; sample size (larvae) is indicated within the plot for each genotype. For each larva, we analyzed responses from a 20-s recording from one muscle 1 NMJ between segments A4-A6. Datapoints represent the time to peak ΔF/F and 50% or 100% recovery. Comparisons were analyzed with a mixed-effects two-way ANOVA. Genotypes are Ib-GAL4, UAS-myrGCaMP6s/UAS-tdTom::Sec61β, in either a WT or Rtnl1¹⁸ background.