Related to Fig. 4 . (A) Time-lapse images of MDM infection by the synapse-like mechanism: MDMs were co-cultured with HIV-GFP-infected Jurkat cells as in Fig. 4 A (see also Video 5). HIV-GFP in green, dashed lines delineate the MDM. Scale bars, 10 µm. Representative images of more than 10 synapse-like contacts. (B) Representative images of F-actin cytoskeleton of MDMs treated different drugs (Jasplakinolide, Cytochalasin D, or CK666) or not (Control). F-actin (phalloidin, gray) and nuclei (DAPI, cyan). Scale bars, 10 µm. (C) Representative images of actomyosin cytoskeleton of MDMs treated different drugs (Blebbistatin, Y27632, or TAT-C3) or not (Control). F-actin (phalloidin, gray), Myosin IIA (red) and nuclei (DAPI, cyan). Scale bars, 10 µm. (D) Same analysis as in (C), except that MDMs were treated with Calyculin A or not (Control). Scale bars, 10 µm. (C and D) These images come from a mosaic (stitched together by the microscope).