Related to Fig. 2 . (A) Ex vivo infection of purified tissue macrophages isolated from first trimester placenta and co-cultured for 24 h with either cell-free virus, or infected Jurkat cells in presence or not of neutralizing anti-CD4 antibody and stained for HIV-p24 (yellow) and F-actin (phalloidin, blue). Scale bars, 200 µm. (B) Macrophages under the same conditions as in (A) stained for HIV-p24 (green), F-actin (phalloidin, red), and nuclei (DRAQ5, gray) and analyzed by confocal microscopy. Scale bars, 10 µm.