Figure 2.
HIV-1-induced heterotypic cell fusion with infected Jurkat T cells is relevant in several human tissue macrophages. (A) In situ tissue macrophage infection: Non-inflammatory human synovial explants were incubated with 2 d-infected Jurkat cells during 24 h and processed for immunohistological analyses. Nuclei (DAPI, gray), HIV-p24 (green), CD3 (CD4+ T cell marker, orange), and CD68 (macrophage marker, red). Scale bar, 30 µm. (B and C) Ex vivo infection of purified tissue macrophages: Macrophages isolated from adult broncho-alveolar lavages (B, see also Video 2) or children hyperplasic tonsils (C, see also Video 3) were co-cultured for 24 h with 2-d-infected Jurkat cells pre-loaded with CellTracker (gray) and analyzed by confocal microscopy. Representative confocal microscopy images with HIV-p24 (green), F-actin (phalloidin, red), and nuclei (DAPI, blue). Scale bars, 10 µm (B) and 20 µm (C).

HIV-1-induced heterotypic cell fusion with infected Jurkat T cells is relevant in several human tissue macrophages. (A) In situ tissue macrophage infection: Non-inflammatory human synovial explants were incubated with 2 d-infected Jurkat cells during 24 h and processed for immunohistological analyses. Nuclei (DAPI, gray), HIV-p24 (green), CD3 (CD4+ T cell marker, orange), and CD68 (macrophage marker, red). Scale bar, 30 µm. (B and C) Ex vivo infection of purified tissue macrophages: Macrophages isolated from adult broncho-alveolar lavages (B, see also Video 2) or children hyperplasic tonsils (C, see also Video 3) were co-cultured for 24 h with 2-d-infected Jurkat cells pre-loaded with CellTracker (gray) and analyzed by confocal microscopy. Representative confocal microscopy images with HIV-p24 (green), F-actin (phalloidin, red), and nuclei (DAPI, blue). Scale bars, 10 µm (B) and 20 µm (C).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal