Related to Fig. 1 . (A) Flow cytometry analysis of MDMs co-cultured with uninfected (NI) or 2 d-infected Jurkat cells (HIV). Left: Representative dot plots of HIV-p24 and CD3 stainings and gating strategy to determine the % of CD3⁺ among HIV-p24 + MDMs. Right: Quantification (n = 11 donors). (B and C) MDM infection after a 24 h-co-culture with uninfected Jurkat cells (NI) or Jurkat cells infected for 2 d (HIV-2d) or 7 d (HIV-7d). (B) Flow cytometry analysis: Representative dot plots of HIV-p24 signals and gating strategy for selection of infected cells. See quantification in Fig. 1 D. (C) Representative images of microscopy analysis. F-actin (phalloidin, blue) and HIV-p24 (yellow). Scale bars, 200 µm. See quantification in Fig. 1 E. (D) Left: Quantification of MDM infection after a 24 h-co-culture with autologous CD4⁺ T cells infected for 2 d (HIV-2d) or 9 d (HIV-9d) (n = 3 donors). Right: Representative images of microscopy analysis of nuclei (DAPI, blue) and HIV-p24 (yellow). Scale bars, 200 µm.