MARK2i does not affect spindle rotation. (a) Illustration shows decomposition of spindle movement (gray) from t₀ to t₁ in 3D. Red dots correspond to spindle poles P1 and P2. The spindle length axis is represented by the black line. Purple arc arrow indicates rotational movement of the spindle length axis at t₁ when parallel to t₀. Blue box: Blue lines cover the longitudinal movement ∆lg along the spindle length axis. Orange box: Orange lines cover the equatorial movement ∆eq along the spindle width axis which is perpendicular to the spindle length axis. Green box: Green lines cover the axial movement ∆ax along the spindle axial axis. The 2D representation (bottom row) illustrates the raw decomposed movement. (b) Polar scatter plot shows spindle rate of rotational movement (in degrees) through time. Measurements at each time point are highlighted with a unique color. α, β, and γ angles refer to spindle tumbling, rolling, and rotation respectively. Statistical significance was determined by Mann–Whitney U test after a pre-analysis of the underlying distribution with a Shapiro–Wilk test. N = 11 control and N = 12 MARK2i cells across three experiments.