CENP-E inhibition promotes 3D spindle rotation. (a) Polar scatter plot shows spindle rate of rotational movement (in degrees) through time. Measurements at each time point are highlighted with a unique color. α, β, and γ angles refer to spindle tumbling, rolling, and rotation. (b) Line plots show average cumulative rate of rotation for spindle tumbling (α), spindle rolling (β), and spindle rotation (γ) against time for control (black) and CENP-Ei (red) inhibited cells, respectively. Fainted lines correspond to individual spindle trajectories. (c) Empirical Cumulative Distribution Function plots show cumulative fraction for longitudinal, equatorial, and axial displacement for control (black) and CENP-Ei (red) inhibited cells, respectively. Fainted lines correspond to individual spindle trajectories. (d) Empirical Cumulative Distribution Function plots show cumulative mean angle values for spindle tumbling (α), spindle rolling (β), and spindle rotation (γ) for control (black) and CENP-Ei (red) cells, respectively. Fainted lines correspond to individual spindle trajectories. Statistical significance was determined by GLM and Kruskal–Wallis test (in c and d) after pre-analysis of the underlying distribution. N = 38 control and N = 43 CENP-Ei cells from three experiments.