Spindle length and width measurements computed through SpinX. (a) Box plots quantifying HeLa SiR-Tubulin and mCherry-Tubulin-labeled spindles length (left) and width (right) as computed through SpinX’s modeling module. Schematics indicate spindle length (blue line) and width (green line) with respect to the detected spindle poles (black and orange dots). (b) Line plots quantifying mean (bold) HeLa SiR-Tubulin and mCherry-Tubulin-labeled spindles length (left) and width (right) across time as computed through SpinX. Thinner lines show measurements from individual cells. Shaded area shows 95% confidence interval. For both a and b, Control (DMSO-treated) SiR-Tubulin N = 82 cells, 5,166 images; MARK2i SiR-Tubulin N = 68 cells, 4,284 images; CENP-Ei SiR-Tubulin N = 43 cells, 2,709 images; Control mCherry-Tubulin N = 44 cells, 2,772 images. Statistical significance determined with Kruskal-Wallis H test and a post-hoc Dunn’s test with Bonferroni correction: ****P < 0.0001; ***P < 0.001, **P < 0.01, *P < 0.05, ns (non-significant). Non-normality was confirmed with a Shapiro–Wilk test.