Figure S7.
Purification of Svf1 from yeast cells. (a) Svf1-FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (b)Svf1V12D-FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (c)Svf1G7AG8A -FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (d)Svf1H273AH274A -FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; E1, eluate 1. (e) Mass photometry analysis of the purified proteins from a–d. Source data are available for this figure: SourceData FS7.

Purification of Svf1 from yeast cells . (a) Svf1-FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (b)Svf1V12D-FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (c)Svf1G7AG8A -FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; W2, wash 2; E1, eluate 1; E2, eluate 2; B, beads. (d)Svf1H273AH274A -FLAG overproduced from the GAL1 promotor was purified via FLAG tag and analyzed by SDS-PAGE. I, Input; FT, flow through; W1, wash 1; E1, eluate 1. (e) Mass photometry analysis of the purified proteins from a–d. Source data are available for this figure: SourceData FS7.

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