Figure 2.
Svf1 dynamically colocalizes with the cis-Golgi. (a) Svf1-GFP was expressed in cells expressing either Mnn9-mKate (cis-Golgi), Sec7-mKate (trans-Golgi), Vps4-3xmCherry (endosome) or HDEL-dsRed (ER). Line scans (right) of the indicated regions of Svf1-GFP (green) and the respective co-expressed organelle markers (magenta). Scale bar = 5 µM. Scale bar inlays = 1 μM. (b) Co-localization of Svf1-GFP and 3xmCherry tagged Mnn9 imaged every 0.9 s over a total time of 9.9 s to confirm the localization to the cis-Golgi. Scale bar = 5 µM. (c) A model of the two interchanging states, cytosolic and membrane bound, of Svf1 in the cell.

Svf1 dynamically colocalizes with the cis-Golgi. (a) Svf1-GFP was expressed in cells expressing either Mnn9-mKate (cis-Golgi), Sec7-mKate (trans-Golgi), Vps4-3xmCherry (endosome) or HDEL-dsRed (ER). Line scans (right) of the indicated regions of Svf1-GFP (green) and the respective co-expressed organelle markers (magenta). Scale bar = 5 µM. Scale bar inlays = 1 μM. (b) Co-localization of Svf1-GFP and 3xmCherry tagged Mnn9 imaged every 0.9 s over a total time of 9.9 s to confirm the localization to the cis-Golgi. Scale bar = 5 µM. (c) A model of the two interchanging states, cytosolic and membrane bound, of Svf1 in the cell.

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