Analysis of SVF1 genetic interactions with other potential ceramide transfer protein. (a) Quantification of tetrad analysis. Relative colony sizes of tetrads of diploid osh2Δosh3Δosh4Δsvf1Δ cells shown as fold change from WT tetrads. Error bars represent standard deviations. (b) Quantification of tetrad analysis. Relative colony sizes of tetrads of diploid osh2Δosh3Δosh4Δnvj2Δ shown as fold change from WT tetrads. Error bars represent standard deviations. (c) Tetrad analysis of svf1Δ (green) mutants crossed with sur2Δ (red). (d) Tetrad analysis of diploid svf1Δydr222wΔylr225cΔsur2Δ cells. svf1Δ (green); ydr222wΔ (blue); ylr225cΔ (yellow); sur2Δ (red). (e) Helical wheel representation of the first 18 amino acids of Svf1. The projection was generated by the Heliquest software (http://heliquest.ipmc.cnrs.fr). (f) Helical wheel representation of the first 18 amino acids of Ydr222w. (g) Helical wheel representation of the first 18 amino acids of Ylr225c.