SVF1 interacts genetically with yeast genes involved in ceramide transport. (a and b) A model for vesicular and non-vesicular ceramide transport in yeast under normal growth and ER stress conditions (b). (c) Histogram of correlation coefficients based on the genetic profiles of yeast mutants in chemical genetic screens. Data was extracted from Hillenmeyer et al. (2008). (d) Tetrad analysis of svf1Δosh3Δosh4Δ (green, yellow, and red, respectively) mutants crossed with nvj2Δosh2Δ (orange and blue, respectively). (e) Serial dilutions of WT, svf1Δ, nvj2Δ, and svf1Δnvj2Δ on YPD plates (control) and YPD plates containing 0.5 µM myriocin.