Figure S3.
MENA and EVL overexpression enhance DF motility. (A) Representative Western blot of protein lysates from D11 mouse cortical neuron cultures expressing EGFP-MENA or EGFP-EVL, and probed with antibodies targeting MENA or EVL for detection of endogenous and overexpressed species. (B) Examples of extreme phenotypes observed in live primary mouse cortical neurons at day in vitro 11 (D11) expressing mRuby2-LifeAct with high expression (SNR > 1.5) of EGFP-MENA or EGFP-EVL. Left: full cell image. Closed arrowheads indicate abnormal structures. Open arrowhead indicates magnified region (right). (C) Examples and quantification of DF morphological phenotypes observed in D11 neurons expressing mRuby2-LifeAct with low-expression (SNR < 1.5) of EGFP-MENA or EGFP-EVL. n = 83–133 DF per condition, N = 3 neurons. Scale bars = 10 µm. See also Fig. 2 and Video 2. Source data are available for this figure: SourceData FS3.

MENA and EVL overexpression enhance DF motility. (A) Representative Western blot of protein lysates from D11 mouse cortical neuron cultures expressing EGFP-MENA or EGFP-EVL, and probed with antibodies targeting MENA or EVL for detection of endogenous and overexpressed species. (B) Examples of extreme phenotypes observed in live primary mouse cortical neurons at day in vitro 11 (D11) expressing mRuby2-LifeAct with high expression (SNR > 1.5) of EGFP-MENA or EGFP-EVL. Left: full cell image. Closed arrowheads indicate abnormal structures. Open arrowhead indicates magnified region (right). (C) Examples and quantification of DF morphological phenotypes observed in D11 neurons expressing mRuby2-LifeAct with low-expression (SNR < 1.5) of EGFP-MENA or EGFP-EVL. n = 83–133 DF per condition, N = 3 neurons. Scale bars = 10 µm. See also Fig. 2 and Video 2. Source data are available for this figure: SourceData FS3.

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