LC3A is not a cofactor for LMX1B transcriptional control of selected genes. (A) qRT-PCR measurements of LC3A mRNA levels following siRNA depletion. mRNA levels normalized to GAPDH. Mean ± SD (n = 3); Student’s t test: ***P < 0.001 vs. siControl. (B) Effect of LC3A suppression on LMX1B-driven FLAT luciferase reporter expression. Scramble, NURR1, pro-insulin, and ULK1 FLAT promoter luciferase assay (LightSwitch) driven by wild-type or Δ308-317 LMX1B-FLAG in HEK293T cells siRNA depleted for siLC3A. Levels are normalized to empty vector control (pcDNA 3.1). Mean ± SD (n = 3); one-way ANOVA followed by Fishers LSD test for planned comparisons: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.