Related to Fig. 6 . CSPP1 stabilized MTs, but there is no change in protofilament length or curvature. (A) Still images from assays with Taxol-stabilized MT seeds in the absence or presence 40 µM Taxol and the indicated GFP-CSPP-L concentrations. Scale bar, 5 μm. See also Fig. 6 A. (B) MT outgrowth from GMPCPP stabilized MT seeds 5 min after flowing in 5 nM GFP-CSPP-L at the indicated tubulin concentrations. The first frame of the acquisition (green) is overlayed with the maximum projections of 5 min acquisition (magenta) illustrating the newly grown MT lattice. Scale bar, 5 µm. (C) Kymograph illustrating MT growth in the presence of 20 nM mCherry-EB3 together with 10 nM mCherry-CSPP-L and 10 nM GFP-CAMSAP3. Scale bars, 2 μm (horizontal) and 60 s (vertical). (D) Quantification of protofilament length, terminal curvature, and total curvature (from tomograms as shown in 1K). Blue, orange, and gray dots (single data points, tomograms), black circle (mean), SD (error bars). **, P < 0.01, n.s., not significant, Mann–Whitney test. Analysis from two independent experiments.