Tex2 KO affects lysosomal functions. (A and B) Representative images of control, control cells treated with BafA1 (400 nM; 2 h; A), or two HeLa Tex2-KO clones (B), expressing mApple-Lamp1-phLuorin (phLuorin in green; mApple-Lamp1 in magenta) with insets. (C–G) Representative images of two HeLa Tex2-KO clones transiently expressing either Halo-Tex2 (blue; C), Halo-Tex2-∆SMP (D), Halo-Tex2-NT (E), Halo-Tex2-∆1-276 (F), or Halo-Tex2-∆PH (G), and mApple-Lamp1-phLuorin with insets. (H) Percentage of phLuorin-positive lysosomes in control (14 cells), Tex2 KO-1 (11 cells), Tex2 KO-1 rescued by either Halo-Tex2 (13 cells), Halo-Tex2-∆SMP (11 cells), Halo-Tex2-NT (14 cells), Halo-Tex2-∆(1-276) (13 cells), or Halo-Tex2-∆PH (11 cells); and in Tex2 KO-2 (11 cells), Tex2 KO-2 rescued by either Halo-Tex2 (12 cells), Halo-Tex2-∆SMP (13 cells), Halo-Tex2-NT (14 cells), Halo-Tex2-∆(1-276) (12 cells), or Halo-Tex2-∆PH (14 cells) in more than three independent assays. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Mean ± SD. (I) Western blots demonstrate the level of Halo-Tex2 and Halo-Tex2 mutants in rescue experiments. Scale bar, 10 μm in the whole cell images and 2 μm in the insets in A–G. Source data are available for this figure: SourceData F7.