Figure S2.
The targeting of Tex2 to tubular ER appears to be independent of ER tubule-shaping proteins. (A–D) Representative images of a COS7 cell expressing GFP-Tex2 (green), ER-tagRFP (blue) and REEP5-Halo (magenta; A), RTN4-Halo (B), Halo-ATL1 (C), and Halo-ARL6IP1 (D) with insets. (E–H) GFP-Trap assays from COS7 cells demonstrate interactions between GFP-Tex2 and Reep5-Halo (E), RTN4-Halo (F), Halo-ATL1 (G), and Halo-ARL6IP1 (H). (I) Representative images of USO2 cells expressing GFP-Tex2 (green), ER-tagRFP (magenta) with insets upon treatments of scrambled, RTN4, REEP5, ARL6IP1, RTN1, and RTN3 siRNAs. (J) qPCR assays showing the efficient of siRNA-mediated suppression in I. Scale bar, 10 μm in the whole cell images and 2 μm in the insets in A–D and I. Source data are available for this figure: SourceData FS2.

The targeting of Tex2 to tubular ER appears to be independent of ER tubule-shaping proteins. (A–D) Representative images of a COS7 cell expressing GFP-Tex2 (green), ER-tagRFP (blue) and REEP5-Halo (magenta; A), RTN4-Halo (B), Halo-ATL1 (C), and Halo-ARL6IP1 (D) with insets. (E–H) GFP-Trap assays from COS7 cells demonstrate interactions between GFP-Tex2 and Reep5-Halo (E), RTN4-Halo (F), Halo-ATL1 (G), and Halo-ARL6IP1 (H). (I) Representative images of USO2 cells expressing GFP-Tex2 (green), ER-tagRFP (magenta) with insets upon treatments of scrambled, RTN4, REEP5, ARL6IP1, RTN1, and RTN3 siRNAs. (J) qPCR assays showing the efficient of siRNA-mediated suppression in I. Scale bar, 10 μm in the whole cell images and 2 μm in the insets in A–D and I. Source data are available for this figure: SourceData FS2.

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