The stabilization of caveolae by Dyngo 4a is independent of Dyn2. (A) Color-coded trajectories of caveolae in Dyn2-GFP-Cav1-mCh cells pretreated with DMSO (Ctrl), Dyngo 4a, or Ryngo 1-23 for 30 min before imaged on TIRF over 5 min. Scale bar, 10 μm. (B) Quantification of percentage of caveolae positive for Dyn2-GFP after 30 min treatment with DMSO (black) or Dyngo 4A (gray). Data from at least 16 cells per condition are shown as scatter dot plot, mean ± SEM. (C) Quantification of Cav1-mCh track duration time and displacement length in cells as indicated. Numbers were related to ctrl-treated cells. Analysis was performed using Imaris software, and track mean from at least eight cells per condition are shown ± SEM. (D) Quantification of Cav1-mCh track duration time in Cav1-mCh cells treated with siRNA as indicated prior to pretreatment of DMSO (Ctrl) or Dyngo 4a as in A. Numbers were related to DMSO-treated ctrl siRNA-treated cells (black). Analysis was performed using Imaris software, and track duration time from at least eight cells per condition is shown as ± SEM. Significance was assessed using t test, ***P ≤ 0.001.