Figure S4.
Overexpression of Dyn2-GFP and K44A Dyn2-GFP, and characterization of protein expression in K44A Dyn2-GFP-Cav1-mCh FlpIn cells and I684 Dyn2-GFP-Cav1-mCh FlpIn cells. (A)Representative images of Cav1-mCh HeLa FlpIn cells transiently expressing wild-type Dyn2-GFP (top panel) or K44A Dyn2-GFP (bottom panel). Scale bar, 10 μm. (B) Immunoblot of K44A Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Doxycycline concentration used for experiments were 1.0 ng/ml. Molecular weights in kD. (C) Representative images of fixed K44A Dyn2-GFP Cav1-mCh FlpIn cells incubated for 10 min with Tf-647 (5 μg/ml) at 37°C. Yellow arrows indicate cells with high expression of K44A Dyn2-GFP and the coinciding lack of Tf-647 uptake. Scale bar, 10 μm. (D) Immunoblot of I684K Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Molecular weights in kD. Source data are available for this figure: SourceData FS4.

Overexpression of Dyn2-GFP and K44A Dyn2-GFP, and characterization of protein expression in K44A Dyn2-GFP-Cav1-mCh FlpIn cells and I684 Dyn2-GFP-Cav1-mCh FlpIn cells. (A)Representative images of Cav1-mCh HeLa FlpIn cells transiently expressing wild-type Dyn2-GFP (top panel) or K44A Dyn2-GFP (bottom panel). Scale bar, 10 μm. (B) Immunoblot of K44A Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Doxycycline concentration used for experiments were 1.0 ng/ml. Molecular weights in kD. (C) Representative images of fixed K44A Dyn2-GFP Cav1-mCh FlpIn cells incubated for 10 min with Tf-647 (5 μg/ml) at 37°C. Yellow arrows indicate cells with high expression of K44A Dyn2-GFP and the coinciding lack of Tf-647 uptake. Scale bar, 10 μm. (D) Immunoblot of I684K Dyn2-GFP-Cav1-mCh cells treated with concentrations of doxycycline as indicated. Molecular weights in kD. Source data are available for this figure: SourceData FS4.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal