Figure S2.
SIM analysis of the colocalization between Dyn2-GFP and Cav1-mCh. (A) Representative SIM2 image of Dyn2-GFP-Cav1-mCh FlpIn cell imaged live. The white line marks the outline of the cell and the dashed square marks the location of the magnified areas in the bottom panel. White arrowheads highlight structures positive for both Dyn2-GFP and Cav1-mCh. Scale bar, 10 or 2 μm for magnification. (B) Orthogonal view of a live Dyn2-GFP-Cav1-mCh cell imaged with Lattice SIM2. Scale bar, 10 μm. (C) Dyn2-GFP-Cav1-mCh cell with line that indicates location of kymograph in Fig. 2 D. Scale bar, 5 μm. (D) Dyn2-GFP-Cav1-mCh cell with line that indicates the location of the kymograph presented at the bottom. Scale bar, 5 or 1 μm for magnification. Insets show TIRF image series of a caveola positive for both Cav1-mCh and Dyn2-GFP that disappear from the TIRF field. Time in seconds as indicated.

SIM analysis of the colocalization between Dyn2-GFP and Cav1-mCh. (A) Representative SIM2 image of Dyn2-GFP-Cav1-mCh FlpIn cell imaged live. The white line marks the outline of the cell and the dashed square marks the location of the magnified areas in the bottom panel. White arrowheads highlight structures positive for both Dyn2-GFP and Cav1-mCh. Scale bar, 10 or 2 μm for magnification. (B) Orthogonal view of a live Dyn2-GFP-Cav1-mCh cell imaged with Lattice SIM2. Scale bar, 10 μm. (C) Dyn2-GFP-Cav1-mCh cell with line that indicates location of kymograph in Fig. 2 D. Scale bar, 5 μm. (D) Dyn2-GFP-Cav1-mCh cell with line that indicates the location of the kymograph presented at the bottom. Scale bar, 5 or 1 μm for magnification. Insets show TIRF image series of a caveola positive for both Cav1-mCh and Dyn2-GFP that disappear from the TIRF field. Time in seconds as indicated.

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