OMM curvature analysis. (A) Representative membrane surface reconstructions of elongated (top) and fragmented (bottom) mitochondria in MEFmtGFP cells treated with vehicle and Tg (500 nM, 8 h). The OMM surface is colored by membrane curvedness and the IMM surface is represented as a transparent gray mesh. (B) Combined histogram of total OMM curvedness of elongated (left) and fragmented (middle) mitochondria in MEFmtGFP cells treated with vehicle and Tg. Dashed vertical lines correspond to peak histogram values of pooled data. Graph (right) showing peak histogram values from each mitochondrion within each corresponding treatment and mitochondrial morphology class. Quantifications from vehicle elongated n = 20, Tg elongated n = 18, vehicle fragmented n = 11, and Tg fragmented n = 15 mitochondria are shown. *P < 0.05; ****P < 0.001.
