Arsenic-induced degradation of the PML/RARA oncoprotein requires p97. (A) NB4 cells were treated with the indicated concentrations of CB-5083 and exposed to arsenic (As) for the indicated time (h). Cell lysates were analyzed by Western blotting using an antibody to PML, RARA, p97, and NPLOC4. (B) NB4 cells were either untreated (−), treated with CB-5083 (CB), bafilomycin (Baf), or bortezomib (Bor). Response to arsenic (6 h) was investigated for all conditions. Cell lysates were analyzed by Western blotting using antibodies to PML, RARA, LC3, or ubiquitin. Molecular weight markers are indicated in kD. Source data are available for this figure: SourceData F7.