Inhibition of p97 alters arsenic-induced changes to PML body number, morphology, and content. U2OS PML^−/− + YFP-PML cells were either untreated or treated with 1 μM arsenic (As), 5 μM CB-5083, or a combination of arsenic and CB-5083 and analyzed by time-lapse fluorescent imaging for 6.75 h. 10 videos were collected for each condition (one video from each condition is shown in Videos 1, 2, 3, and 4). (A) Stills from the start (0 h) and the end (6.75 h) of the movies. Scale bars are 20 μm. (B) The average PML body size, number of PML bodies per cell, total area of PML bodies, and total intensity of PML bodies was plotted as a function of time (n = 10 fields of view). (C) Comparison of relative PML intensity per cell after 6.75 h treatment as indicated. Columns represent average and error bars are 1 SD. P values are two-tailed student t tests assuming equal variance or unequal variance (*). Each data point is derived from a single field of view taken from the time course shown in B (n = 10). Source data are available for this figure: SourceData F3.